About us
AquaBioSens develops handheld devices to measure aquatic hazards and pollution, supporting the EU Mission to “Restore our ocean and waters by 2030”. The devices use novel analytics, such as immunoassays, environmental RNA quantification, and whole cell biosensors, coupled with advanced sensors, such as acoustic biosensors, fluorimetry, and organ-on-chip devices. The devices are low-cost, accessible, and connected to the web. They will be tested and validated in coastal and freshwater sites in the UK, Ireland, and Greece, and disseminated to the international community. AquaBioSens aims to commercialize the new technologies.
Duration
01/2024 to 12/2026
 
Programme
HORIZON EUROPE Framework Programme
HORIZON-CL6-2023-ZEROPOLLUTION-01
 
Reference
101135432

WP1: Device Design and farbrication

Task 1.1 Organic contaminant and algal toxin concentration and extraction

Task 1.2 SAW device for organic contaminant immunoassays

Task 1.3 Fluidic device for cell lysis and mRNA preconcentration

Task 1.4 Device for isothermal real-time RNA amplification and multi-channel fluorescence detection

Task 1.5 Fabrication of microfluidic device for fish gill cell epithelia growth and TER measurements

Task 1.6 Smartphone apps for device operation

WP2 Immuno-SAW sensing for organic contaminants and biotoxins

Task 2.1 Recombinant antibody development towards bifenthrin

Task 2.2 Antibody assay development and optimization

Task 2.3 Biofunctionalization of the device surface and acoustic immunoassay

Task 2.4 Optimise solvent concentrations for SAW assays

Task 2.5 Optimization with simulated samples in laboratory

WP3 Environmental RNA sensing for harmful microbes and biotic pollution

Task 3.1 Identify target genes for assays

Task 3.2 Develop RNA amplification assays with stored reagents and crude samples

Task 3.3 Develop cell lysis and selective mRNA preconcentration

Task 3.4 Optimization with simulated samples on the eRNA analyzer device

WP4 Fish gill whole cell metal biosensing on chip

Task 4.1 Fish Gill Cell culture System

Task 4.2 Zn toxicity effects on membrane integrity

Task 4.3 Zn toxicity assay with fluorescence read-out

Task 4.4 Metal toxicity assays with gene expression read-out

Task 4.5 Testing with simulated samples

WP5 Diatom whole cell biosensors for multiplexed heavy metal quantification

Task 5.1 Identify P. tricornutum and T. pseudonana cadmium and nickel responsive promoters

Task 5.2 Clone genetic elements and test reporter genes performance in episomal P. tricornutum and      T. pseudonana modular, extrachromosomal expression libraries

Task 5.3 Assess biocontainment of the diatom Cd/Ni WCBs

Task 5.4 Test performances of the diatom Cd/Ni WCBs on the multi-channel fluorescence detection hand-held device in laboratory conditions

WP6 Field testing of technologies

Task 6.1 Field testing of the Immuno-SAW device

Task 6.2 Field testing of the eRNA analyzer

Task 6.3 Field testing of fish gill WCB device

Task 6.4 Field testing of diatom WC sensing

WP7 Digital real-time data feeds

Task 7.1 Software architecture definition

Task 7.2 Software development

Task 7.3 Test and data public release

WP8 Dissemination, Exploitation and Communication

Task 8.1 Develop a project info website

Task 8.2 Create a dissemination, communication and exploitation

Task 8.3 Provide digital promotion materials, news articles and create web advertising banners to promote AquaBioSens

Task 8.4 Definition of the long-term pathways to impact and creation of an exploitation plan

Task 8.5 Participate in relevant Biotechnology, Environmental & industry networking events, webinars,   fairs, expos and conferences to promote AquaBioSens

Task 8.6 Create public “policy briefs” documents

WP9 Management

Task 9.1 Establish the project management group

Task 9.2 Arrange and report on all project meetings

Task 9.3 Management of project risks

Task 9.4 Report production

Task 9.5 Data management plan (DMP)

Task 9.6 Synergies with other funded projects